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Minimum Sequences
                  (BMC Bioinformtics 2001, 2:1)

• "For the most-typical codon, the expected R:S is 1:3, >= 3 mutations required"
  
• "For cta or ctg, only 2 mutations required"
•  For negative selection detection, much more mutatations required (> 10)
  

False-positive Testing
                         (BMC Bioinformtics 2001, 2:1)

• Conduct simulation by generating 10 sets of  300 sequences of length 999 (EVOLVER (PAML) )
• The average branch length of each set varies in 0.1 intervals from 0.1 to 1.0
• False positive persentage is calculated and plot against average branch length

                                                                                                                                     derived from Dr. Jeffrey J Stewart, 2001                                                                                                                                                                                                                                                                                                                                                                                                
False positive percentage (F) = false psoitives / (false positive + neutral drift variants)

• F <~2%, if branch lengths are in range of 0.1 --- 1.0
• F >~70%, if branch length is at 0.001