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  Laboratory of RNA Biology
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Xie Lab


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Alternative Splicing

in Nucleic Acids IMED 7240

Research topics in Physiology: Regulation of alternative splicing

PHGY7030

Adrenals/Stress and Stress Hormones

in PHGY 1030, 5006 and 7256, and PAEP7002

Neoplasia

in PAEP7000

Cell Biology B

IMED 7094



Questions: xiej@umanitoba.ca

Office Hours: By appointment


Keywords:
 molecular biology, RNA, processing, alternative splicing, cryptic splicing, transcriptome integrity, hnRNP, gene regulation, gene expression, evolution, proteomic diversity, complexity, splicing factors, protein kinases, cell signaling, neurons, endocrine cells, SNP, mutation, cancer, GWAS, human health/genetic diseases


Highlights of some of the findings by the lab:

hs3ssbar


BP
Splice Site Matrices of >1000 Species/lineages

REPAG_GenomesR38R91


 REPAG
(Right-click image to view details)

The REPAG element specifically among mammals
(Sohail M., and Xie J. Molecular & Cellular Biology, 2015, 35(12): 2203-2214)

Adaptive splicing
(Right-click to view details)
Adaptive splicing

CaRRE
(Right-click image to view details)

The CaRRE element among vertebrates (Liu GD, et al., J. Biol. Chem. 2012, 287:22709–22716)

STREX
                                    model
Molecular basis of the depolarization-regulated alternative splicing of the STREX exon of the Slo1 gene

hnrnpl-prolactin
Importance of proper splicing control in hormone production: aberrant splice variant of prolactin (right) due to the loss of hnRNP L

REPA

A new group of introns: REPA element 'inserted' between the Py and 3'AG and its effects on alternative splicing

hREPAG
Consensus of 1000 human REPAG intron ends (Credit: Aydan Wang)


*Research in this lab has been supported by CIHR , NCIC, CBCF, NSERC, MMSF, MHRC and CFI.


Misce. links:



Faculty of Graduate Studies

Department of Physiology & Pathophysiology

College of Medicine

Faculty of Health Sciences

University of Manitoba